Inhibition of CYP27B1 and CYP24 Increases the Anti-proliferative Effects of 25-Hydroxyvitamin D3 in LNCaP Cells

Background/Aim: Growing evidence suggests that vitamin D3 exerts anticancer effects. The present study aimed to evaluate 25-hydroxyvitamin (25(OH)D3) as a potential endocrine factor regulating proliferation and D receptor expression in LNCaP prostate cancer cells. Materials Methods: Cell counting after treatment was utilized assess the effect of 25(OH)D3 on cell proliferation. Changes mRNA receptors, VDR PDIA3, were evaluated using droplet digital polymerase chain reaction (ddPCR). Results: inhibited dose- time-dependent manner. inhibitory potentiated inhibition CYP17B1 CYP24 by genistein, preventing further metabolism 1,25-dihydroxyvitamin (1,25(OH)2D3) 24,25-dihydroxyvitamin (24,25(OH)2D3). Expression PDIA3 increased with 25(OH)D3, whereas ratio between remained unchanged. Conclusion: has direct proliferation, which is enhanced accelerated when 1,25(OH)2D3 24,25(OH)2D3 inhibitor genistein. Furthermore, transcript expression, suggesting an stability sensibility treated